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O-GlcNAc Stripper (GST-tagged)


Recombinant catalytically active Clostridium perfringens NagJ is a bacterial ortholog of the human O-GlcNAcase. It efficiently and effectively removes O-GlcNAc from peptides, proteins and cell lysates as well as from the artificial substrate 4-methylumbelliferyl-D-glucosaminide. This protein carries an N-terminal GST tag for easy immobilisation.

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  • F.V. Rao, H.C. Dorfmueller, F. Villa, M. Allwood, I.M. Eggleston and D.M.F. van Aalten, "Structural insights into the mechanism and inhibition of eukaryotic O-GlcNAc hydrolysis", EMBO J (2006), 25, 1569–1578. [PubMed] [Reprint]

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